新型线性肌动图 Wire-Myograph from MDE GmbH

发布时间：2020-11-21

新型线性肌动图 Wire-Myograph from MDE GmbH

MDE GmbH - SMALL VESSEL WIRE MYOGRAPH SYSTEMS

1. 我们的小型容器Myograph系统是由原来的Mulvany设置设计的，这意味着系统的加热是通过热水循环来保证的。与其他电加热设备相比，这提供了更多的热稳定性-器官室的准确性和系统中的无噪音记录。这意味着该设备必须与我们在您的报价中包含的CWB一起操作。

2. 我们使用高(x10,000)放大和非常硬(50g)力测量头，这使得系统更加耐用和刚性。

3. Myograph浴槽可从底板上拆卸，以便在显微镜下轻松放置，也便于安装容器环。

4. 我们可以提供不同体积的容器环室，原来我们提供10ml体积浴

5. 校准是由一个特殊的工具包(我们在基础设置中提供)进行的。这使得校准容易和可重复。

6. 我们提供自己的数据采集系统，称为Kymograph，但我们的系统是一个开放的系统，因此它也可以与任何其他DAQ系统一起操作。在我们的报价中，我们在预安装的工作站(计算机)上添加了Kymograph。

Small vessel wire myograph systems

Small vessel myographic system

The system shows a unique modularity with features as follows:

Rapid extension to 1-4 channels,
Removable organ bath (with 2, 5, 10, 20 mL capacity),
Isometric measuring sensor,
Two sets of vessel holders, depending on the diameter of the vessel being examined (Ø60 µm – Ø3 mm),
Gas controller, with a freely positioned diffuser tip,
Continuous temperature control as per request,
Covering lid against evaporation and heat loss,
Locking of the vessel holder to facilitate positioning of the organ,
The measuring unit (organ bath, manipulator, force transducer) can be unlocked and removed from the base plate,
Field-stimulation electrodes (as per request),
Temperature stabilization: circulating water bath thermostat.

Scheme of the system

The system consists of five main units:

Organ bath (flow-through/stationary) with buffer in-and outflow stubs (gravitational or pumped flow) gas controller with freely positionable diffuser tip, and field-stimulating electrodes (as per request). Vessel holders: tungsten wires or steel prods (depending on vessel lumen); vessel holder locking for positioning of the organ,
In- and outlet stub of the thermostated circulating water bath (20 l, 15 l/min flow rate),
Precision manipulator (0.25 mm/turn),
Isometric sensor,
Base plate with lock/unlock lever for the measuring unit.

Organ bath, measuring unit

Organ bath

One-module system with microscope

The organ bath is manufactured in four different volumes (2, 5, 10, 20 ml). The standard volume is 5 ml unless requested otherwise. Each volume is available for later order, and can be replaced in the measuring unit.

The tubes carrying gas and solution for maintaining optimal physiological environment are connected detachably to the measuring unit through connectors fixed in the base plate.
Removable design of the measuring unit greatly facilitates placement of the preparation in the vessel holder because the unit can be put on the stage of a microscope.

Removing the measuring unit

The organ bath, the tension manipulator and the sensor are built into one unit which can be removed, for the sake of easy handling, in the following way:

Unit locking	1. Turn the lever locking the unit to the base plate and tube connector in 90O angle	Measuring unit and baseplate
Unit sliding	2. Slide out the unit along the guide rail of the base plate	Measuring unit and baseplate

Vessel holders, precision setting of gas supply

The tungsten wire vessel holder type ISO-12-21 is recommended for vessel preparations with an inner diameter between 0.05 mm and 0.5 mm, and length up to 3.5 mm.The choice of vessel holders is determined by the inner diameter and length of the preparation. The decision finally depends on the professional competence of the person performing the experiment. The vessel holder can be interchanged in the sleeves, within and between types.

Wire-type vessel holder

The steel prod vessel holder type ISO-12-22 can be used also for preparations with an inner diameter between 0.8 mm and 3 mm, and length over 3.5 mm. This technical solution enables the continuous examination of larger vessels in a small volume of buffer.

Steel spike-type vessel holders

Gas controller and diffuser

Gas vaporizer

Gas controller

For proper working with the preparation, it is vital to have the necessary amount and adequate quality of carbogen gas evenly distributed in the solution.
To ensure the sufficient dispersion, a bubbler and fine-tuner device has been developed. The gas coming through the pressure reducer on the gas cylinder is kept in a gas bag. From there, the gas flows in a sufficiently thick-walled tube through a cylindrical clamp and is applied into the solution via a tube-tip gas diffuser.

Calibration

Parts of the calibration unit

Calibration is done by means of an independent unit which can be connected to the system.

Parts of the unit:

Holder with the pulley with bearing,
Thread with hook,
1g certified weight.

Calibration

Steps of the calibration:

Remove the precision manipulator and replace it with the pulley holder,
Lock the isometric sensor with the lever. Hitch the thread to the stub on the vessel holder sleeve of the sensor, then lead it over the pulley,
Attach the 1 g calibration weight to the hook of the thread,
Unlock the sensor by turning the lever back in 90O.

Gravitational flow

Pumped flow

Accessories required for operation

ISO-12W Workstation

The analog amplifiers, the computer and the software constitute an autonomous, integrated unit in the workstation. This solution allows fast configuration, training and promotes concentration on the experimental work.

The following hardware-software systems are recommended to graphically display, store and analyze the measured physiological parameters: