非侵入性地记录神志清楚的小鼠、大鼠和荷兰猪的心电图。
System to Non-invasively Record Electrocardiograms in Conscious Mice, Rats, and Guinea Pigs
ECG Measurements in Concious Mice.pdf
The ECGenie is a non-invasive electrocardiogram recording system. An easy method of recording ECGs in conscious mice. Provides a lead II ECG from the paws. Records the HR, P-R, QRS, QT intervals and shows a power spectral graph.
Applications would include arrhythmia detections, health screenings and drug effects.
The ECGenieTM is the only truly non-invasive instrument for cardiac function in the conscious ambulatory lab animal. One system is capable of measuring ECGs in large quantities of rodents. The operator simply places an animal onto the recording platform, allowing sufficient time for the animals to acclimate, and triggers recording when the paws of the animal are in contact with the electrodes. One technician can record ECGs in approximately 10 subjects each hour. With ease and speed you may be able to non-invasively detect the sought-after cardiovascular phenotype or identify drug-induced arrhythmias in your conscious mice, rats, and guinea pigs.
UNLIKE TELEMETRY DEVICES OR CATHETERS, THE ECGenieTM SYSTEM FOR NON-INVASIVELY RECORDING ECGS IN CONCIOUS RODENTS DOES NOT REQUIRE ANESTHETIC OR SURGERY.
Applications include:
- Phenotyping
- Genotyping
- Health Monitoring
- Neonatal Electrocardiograms
- Arrhythmias
- Cardiac hypertrophy
- Cardiovascular studies
- CNS studies
- Developmental studies
- Endocrine studies
- Metabolic studies
- Preclinical study of new compounds
Data include:
- Heart rate
- Heart rate variability
- RR interval
- PR interval
- QRS interval
- QT and QTc intervals
The method and system have now been described in over 15 publications.
ECGenieTM ECG Screening system for non-invasively recording ECGs in conscious mice.
1. Chu V, Otero JM, Lopez O, Morgan JP, Amende I, Hampton TG. Method for non-invasively recording electrocardiograms in conscious mice. BioMedCentral Physiology 2001, 1:6.
2. Chu V, Otero JM, Lopez O, Sullivan M, Morgan JP, Amende I, Hampton TG. Electrocardiographic findings in mdx mice: a cardiac phenotype of Duchenne muscular dystrophy. Muscle & Nerve 2002;513-519.
3. Min JY, Sullivan MF, Yan X, Feng X, Chu V, Wang JF, Amende I, Morgan JP, Philipson KD, Hampton TG. Overexpression of Na+/Ca2+ exchanger gene attenuates postinfarction myocardial dysfunction. Am J Physiol Heart Circ Physiol 2002 Dec;283(6):H2466-71.
4. Sutliff RL, Haase C, Russ R, Hoit BD, Morris R, Norman AB, and Lewis W. Cocaine Increases Mortality and Cardiac Mass in a Murine Transgenic Model of Acquired Immune Deficiency Syndrome. Lab Invest ; 2003; 83: 983.
5. Braun A, Trigatti BL, Post MJ, Sato K, Simons M, Edelberg JM, Rosenberg RD, Schrenzel M, Krieger M. Loss of SR-BI Expression Leads to the Early Onset of Occlusive Atherosclerotic Coronary Artery Disease, Spontaneous Myocardial Infarctions, Severe Cardiac Dysfunction, and Premature Death in Apolipoprotein E.Deficient Mice. Circ Res 2002; 90: 270-276.
6. Braun A, Zhang S, Miettinen HE, Ebrahim S, Holm TM, Vasile E, Post MJ, Yoerger DM, Picard MH, Krieger JL, Andrews NC, Simons M, Krieger M. Probucol prevents early coronary heart disease and death in the high-density lipoprotein receptor SR-BI/apolipoprotein-E double knockout mouse. Proc Nat Acad Sci 2003; 100:7283-7288.
7. Svenson KL, Bogue MA, Peters LL. Invited review: Identifying new mouse models of cardiovascular disease: a review of high-throughput screens of mutagenized and inbred strains. J Appl Physiol 2003 94:1650-9.
8. Piskorksi K, Kale A, Chu V, Otero JM, Gladstone K, Mueller P, Amende I, and Hampton TG. Non-invasive physiology in conscious mice. 2002 Fourth World Congress Alternatives Congress Trust Proceedings. 2004, in press.
9. Amende I, Dubach JM, Piskorski K, Morgan JP, and Hampton TG. ECG Monitoring and Analyses in Mice. The Physiological Genomics of the Critically Ill Mouse in Basic Science for the Cardiologist Published by Kluwer Academic Publishers on mouse cardiovascular physiological genomics. 2004. in press.
10. Schuldt AJT, Hampton TG, Chu V, Vogler CA, Galvin N, Lessard ML, Barker JE. Cardiac anomalies in beta-glucuronidase (GUSB) null mice are corrected by non-ablative neonatal marrow transplantation. Proc Nat Acad Sci 2004.