|
Alembic Instruments 隆重推出新型全细胞电压膜片钳放大器
----VE-2 Patch Clamp amplifier
The world's fastest whole-cell voltage clamp amplifier!
Eliminates series resistance errors !
For the fastest, largest ionic currents, you need the VE-2
Advantages 最好的性价比(是同类进口产品价格的1/3) 及其技术优势
· The only amplifier that fully compensates for series resistance and eliminates series resistance errors;
它是唯一的完全地补尝串联电阻并且消除串联电阻错误的放大器
· Voltage Clamp the largest, fastest whole-cell currents under physiologic conditions.
在生理状况下,它能获得最大的电压钳位和最快速的全细胞电流
· Super stability gives super ease-of-use. 易常的稳定使得操作极其容易。
· Just 2 controls quickly and easily sets compensation. 仅有的二个控制键使得设置代偿更加快速和容易。
· Familiar IV converter architecture has all the features you expect. 与IV交换器一样有您期望的所有特点。
Only the VE-2 can compensate fully for Rs (C). (Sherman et. al., Biophys. J., 77:2590-2601, 1999.)
There is no voltage error after ~ 200 µs in every cell you voltage clamp, regardless of the current amplitude !
And the VE-2 is stable even when over compensated.
Record currents that were out of reach until now.
Record from the largest cells.
Finally, beat series resistance.
| The series resistance challenge |
|
Other amplifiers can only compensate
~ 90 % of series resistance (Rs).
In demanding applications, Rs voltage errors of only a few millivolts cause uncontrolled current activation and loss of the voltage clamp.
(Eg. Ina in neurons or cardiac myocytes)
Other amplifiers can partially compensate for Rs (A), but become unstable when pushed too far (B).
With other amplifiers, even 95 % Rs compensation is insufficient to voltage clamp and measure large ionic currents. |
|
|
|
Cell voltage during a membrane ionic current pulse using 90 % (A) and 97 % (B) Rs compensation found on other amplifiers. The voltage error is reduced to 5mV (B) but undampened oscillations indicate instability.
Rs=10 MOhm, Cm=50pF, Ipulse=20nA, Bandwidth=50kHz. | |
|
The_ VE-2 solution...
Only the VE-2 compensates it all |
|
Only the VE-2 can compensate fully for Rs (C). (Sherman et. al., Biophys. J., 77:2590-2601, 1999.)
There is no voltage error after ~ 200 µs in every cell you voltage clamp, regardless of the current amplitude !
And the VE-2 is stable even when over compensated.
- Record currents that were out of reach until now.
- Record from the largest cells.
- Finally, beat series resistance.
|
 |
|
Cell voltage during a membrane ionic current pulse using VE-2 Rs compensation, showing no voltage error after ~ 200 µs.
(Parameters same as above) | |
Recent Publications (Using VE-2 Amplifier):
|
