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Multiwavelength Fluorescence Photometry
[ 作者:佚名 | 来源:本站原创 | 点击数:2500 | 更新时间:2007-08-02  ]
新产品: 美国 C & L Instruments多光谱荧光离子浓度测定系统--定量荧光测定
[Ca++],[Na+],[Mg++], [Cl-],PH & 膜电位
 

 
美国 C & L Instruments多光谱荧光离子浓度测定系统  
 
       
伴随着计算机技术和光学技术的发展,在生命科学领域里,对机体现象应用荧光光度测定及数据处理的方法,正随着新的萤光试剂的开发变得越来越普及。  经过二+年的不断创新,美国C & L Instruments 推出了当今世界上最先进的,最好的性价比的荧光光度测定系统, 它具有激光扫描共焦显微镜在细胞定量荧光测定和细胞内钙离子PH值及其它离子的动态分析的特点、还可在不用显微镜情况下进行细胞或组织定量荧光测定. 价格是同类进口产品的2/3,  是激光扫 描共焦显微镜的1/10。
 
 
技术优势:
 
  • 高精密度可计算机调控的8波长激发光 和可计算机调控的8波长辐射接收光系统;
  • 数控 激发光的强度
  • 扫描速度快, 1000次/ 秒,
  • 高精分辨系统;
 
自从1988年此系统问世以来,它己被全世界100多家顶级医学科研机构和大型药厂的研究院广泛采用。这是一个具有划时代意义的系统,它的特点是高度精密,容易操作,实验结果可靠, 且具权威。
 
系统由以下部分组成:
 
  • 高精密度可计算机调控的8波长的激发光源;
  • 全新设计的光电倍增管和可计算机调控的8波长的辐射接收光收集处理系统;
  • 数椐处理和转换中心;
  • 细胞培养器及其温度自动调节系统       
  • 完美的计算机资料摄取及其分析软件。
 
高速钙离子荧光测量系统的原理:
 
采用钙离子荧光探针对细胞内钙离子等信使物质进行定量测定是研究细胞活动的重要技术手段。其基本原理是:用荧光探针标记样本中的钙离子,根据样本中的荧光探针特性单色光源发出单色光,诱发出荧光。然后根据传感器检测到的荧光特性即可分析样本中的钙离子浓度。
测量时一般采用单波长法和双波长比率测量法,因双波长比率法的灵敏性和方便得到更广泛的应用,其原理是:探针与钙结合后不仅产生荧光强度的变化,而且有激发光或发射光谱偏移。激发峰偏移者进行双激发比例荧光测量,如fura-2; 发射峰偏移者进行双发射比例荧光测量,fura-2的340/380,可获得最大的比值。  应用荧光光度测定系统Model S48D Illumination Source、 荧光显微镜系统 , 光电倍增器检测  Model D48 Detector, 即可获得校准的细胞内Ca++浓度。
 
还有其他一些指示剂在不同的实验中也得到了应用。
Fura-2:双波长340/380nm激发;单波长510nm Ca++测量
Fura-3:单波长490nm激发;单波长530nm Ca++测量.
Indo-1:单波长350nm激发;双波长405/480nm pH测量;
BCECF:双波长430/480nm激发;单波长530nm pH测量;
MQAE: 单波长352nm激发;单波长450nm Cl- 测量.
 
荧光测钙系统可分为:
1.单色光源 Model S48D Illumination Source,
实现ms级激发光波长的转换、
2. 荧光显微镜、3.荧光采集系统(光电倍增管) Model D48 Detector 
此系统中单色光源提供单色激发光,随后将激发光引入荧光显微镜;荧光显微镜将激发光聚焦于样本平面以诱发荧光;荧光采集系统采集并记录荧光信号。计算机控制 单色光波长的切换、荧光信号的采集以及实验数据的初步处理。
 
 
应用:
  1. 神经生物学:离子浓度改变引发的信号发射
  2. 生理学:肌肉运动-心肌细胞中的钙信号
  3. 细胞生物学:信号传导;离子通道
  4. 发育生物学:卵受精机制
  5. 药物学:筛选药物、药效学考察等方面
 
 
Multiwavelength Fluorescence Photometry Systems
 
Overview
We off several types of fluorescence photometry systems for inverted microscopes.  If you have an standard upright microscope, several of these systems may also be used.  
 
The components that make up a complete system can be configured in a manner to match your requirements and your budget.  A simple and inexpensive system consists of a controller (PC-DAQ) and the D48 Detector (System 1).  Using your existing microscope illuminator, this allows to quantitate fluorescence intensity using up to eight emission wavelengths.  The addition of a filter changer (Model MFW-4) allows you to automate the selection of the excitation wavelength without moving the microscope dichroic cube (System 2 and 3).   Finally, addition of our S48D Illumination Source provides a Xenon arc source for fully automated multiwavelength excitation (System 4). 
 
These components can also be used to measure fluorescence in cuvettes and with the addition of our cuvette accessory (Model CV1).  The addition of a camera allows multiwavelength and ratio imaging.
C&L's design allows for:
  • High speed and multiwavelength capability.  The Dye Fluorometer can measure eight separate measurements at eight wavelengths in eight milliseconds and display the data in real time.
  • Optical purity in the selection of excitation and emission wavelengths, resulting in very low background signals.
  • The ability to use combinations of dual wavelength excitation and dual wavelength emission dyes. Two or more dual wavelength dyes can be monitored in the same experiment.
  • Software control of illumination intensity.  Illumination intensity can be precisely varied over a 50-fold range for any wavelength pair.
  • Simultaneous acquisition of 8 channels of analog data to collect signals from other instruments. This eliminates the need for collecting other data with secondary recording devices and the difficulty in equating secondary events to changes in fluorescence.
  • Low cost of ownership.  Get into multiwavelength, high speed fluorometry for much less than you would pay for less versatile instruments from other manufacturers.
 
 
 
 
 
 
 
 

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