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Muromachi动物脑内酵素瞬间灭活系统
[ 作者:佚名 | 来源:本站原创 | 点击数:3237 | 更新时间:2010-11-24  ]
 

动物脑内酵素瞬间灭活系统

Muromachi Microwave Fixation system

 

微波灭活系统通过一个专门的发射器将产生的微波能量传递给大鼠或者小鼠的脑部, 使动物大脑内的化学物质快速固定,脑内的酶等化学物质维持不变,使得研究在活体条件下进行。系统能精确测量 cAMP, cGMP, GABA, Acetylcholine, choline, DOPA, 5HTP, endorphin, prostaglandins, and catecholines 以及它们的代谢物。固定大脑的速度与受试动物的身体尺寸有关,小鼠大约一秒钟,大鼠则需要更长一点时间。 该系统有两个型号可选:5千瓦型和10千瓦型。     MMW-05 

 

 工作原理:

1. 大功率微波加热的方式使得神经组织内的酶(ENZYME)瞬间去活性-灭活,以避免死后变化
 
post-mortem changes)持续进展。
2.
此法比用冷冻固定法要来得快速:冷冻法需要10-90秒做深入冷冻去活性,这期间,深部组织的死后变化    已经在进行,因脑表面 的热传导转差。微波法可在更短的时间内使组织完全去活性。
3.
用以测定:      *  Acetylcholine乙酰胆碱
                       
  *   Choline 胆碱
                       *  Cyclic AMP
环腺苷酸
                            
Cyclic GMP 环林鸟苷
                       *  GABA
  伽马氨基丁酸              
                            
DOPA 多巴(二羟基苯丙氨酸)
                       *  5-HTP 5-
羟色胺酸
                       *  Serotonin
血清素
                       *  Endorphin 
内啡肽
                       *  Prostaglandin 
前列腺素
                           *   
Catecholamines and their metabolites 儿茶酚胺及其代谢物
                       *  Phosphorylated Proteins
磷酸化蛋白质

微波灭活固化可以达到下列标准:

1.
可瞬间将温度升至75-90摄氏度,使脑部快速固定。
2.
大功率微波能量可有效集中在动物的头部。
3.
各种动物都可以达到相同的结果。
4.
对操作人员具备严格的保护,设备相当安全。
5.  CE
认证,国际规定: 家用微波设备微波泄漏不得超过5毫瓦/立方厘米,
    
此设备安全标准远远高于国际规定,微波泄漏不超过1毫瓦/立方厘米。


产品特性:


1. 所有控制均位于前触摸屏幕板上;
2.
数字化设置, 使得电源级别设置容易,重复性好;
3.
稳压控制: 很好的控制客户处电压不稳, 电压波动性得到控制;
4.
采用风冷装置,无需水冷,升降温时间快;                                                                        

5.  整体重量轻,仅为103公斤.

                                                                                                  

规格

振荡器

Panasonic Magnetron 2M265-M11

微波输出

5kW max. 2-5 千瓦变频)

震荡频率

2.450±30MHz

冷却要求

空气冷却

水流量

 每分钟两升

暴露时间

0.01-9.99 (0.01秒步进)

暂停时间

3 分钟

能量功耗

8 千瓦  

频率

50 或者 60 Hz

电源要求

190-240 VAC 30A 3 或者
380-440 VAC 20A 3

外形尺寸

750 W x 550 D x 1,275 H 厘米

重量

大约 103公斤



APPLICATOR HEAD   
适用的动物管

TAW-174P

WJM-24 for Mouse 15-20g
WJM-28 for Mouse 20-40g

TAW-424SP

WJR-S for Rat 150-250g

TAW-424MP

WJR-M for Rat 250-400g
WJR-L for Rat 400-500g

发表文献:

Unambiguous assignment of the H3S and H3R deuterations of cerebral (2-13C) glutamate by 13C NMR at 18.8 tesla. TB Rodrigues, IR Violante, S … - Magnetic Resonance in …, 2010 - interscience.wiley.com
... At the end of the infusions, cerebral metabolism was arrested using a 5- kW microwave
fixation system, the brain being rapidly removed from the skull and immediately frozen in liquid N2. ...

Brain glutamine synthesis requires neuronal-born aspartate as amino donor for glial glutamate formation        B Pardo, TB Rodrigues, L Contreras, M … - Journal of Cerebral …, 2010 - nature.com
... regulated circulating water bath. Fifteen minutes after 13 C-label administration, the
metabolism was arrested using a high-power (5 kW) focused microwave fixation system
The brain was ...

Delayed Increase of Brain Noradrenaline After Acute Footshock Stress in Rats

T Shinba, N Ozawa, M Yoshii, K Yamamoto - Neurochemical research, 2010 - Springer
... 4.5–5 kW, 1.4 s) using a small animal microwave applicator...
Immediately after microwave radiation, the brains were harvested and put onto an ice-chilled
Petri dish ... Moroji T, Takahashi T, Ogura K, Toishi T, Arai S (1977) Rapid fixation of rat ...

Altered Affect, Monoamine Transmitters and Bioenergetic Homeostasis of Alpha-synuclein-transgenic Mice, in the Presence and Absence of Endogenous Alpha- EM Cumyn - 2010 - tspace.library.utoronto.ca
... 36 3.7 Open field test..... 37 3.8 Microwave fixation and dissection of brain tissue.....
37 3.9 Fresh dissection and flash-freezing of brain tissue..... 40 ...

Brainstem Concentrations of Cholesterol are not Influenced by Genetic Ablation of the Low-Density Lipoprotein Receptor

AY Taha, CT Chen, Z Liu, JH Kim, HTJ Mount, … - Neurochemical …, 2009 - Springer
... At 7 weeks of age, the mice were euthanized with a high-energy head-focused microwave
fixation system (6 kW, 0.88–0.99 s, Muromachi brain fixation system in order to stop brain lipid metabolism [14, 15]. ...

Detection of reduced GABA synthesis following inhibition of GABA transaminase using in vivo magnetic resonance signal of [13C] GABA C1

J Yang, C Johnson, J Shen - Journal of neuroscience methods, 2009 - Elsevier
... After in vivo MRS data acquisition of gabaculine-treated rats infused with [2,5- 13 C 2 ]glucose
and, unlabeled glucose, the rats were euthanized using a microwave fixation device (Model
TNW-6402C, 5 kW, 1.5 s). Subsequently, the rat ... 

 

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