世界第一个, 也是唯一一个不需预热, 非侵入性新生小鼠和大鼠血压监测仪

-日本MuromachiKikai新品推荐

Capable of recording from neonatal mice (1.4 g) to aged rats (1 kg)

Muromachi MK2000ST   MK1030，不同于传统的大小鼠无创血压计，在室温23℃以上就可以测量 老鼠的血压，很适合用来调查药物对动物的影响，一旦动物在限制管内有安置好，则只要按下 ·            开始键，HR心率(Heart Rate), SB收缩压(Systolic BP), MBP平均血压(Mean BP); DBP舒张压 ·             (Diastolic BP, calculated value)就可以自动测量。

MK-2000ST  不需预热、非侵入式无创大小鼠血压计 产品主要特性 1. 高灵敏度:一般很难量到血压的colored mice (如 C57 BL/6)，仓鼠(麻醉)及天竺鼠(无麻醉)， 使用 Muromachi MK-2000ST都可以测得到其血压。 2. 测量范围大: 新生小鼠(1.4g)-- 成年鼠(1kg)都可以测到其血压。 患病动物也可测量（例如心肌梗塞的动物） 3. 测量老鼠尾部血压不需加热: 室温23°C以上即可。其它传统测量, 加热的过程或 限制动物移动的行为，可能会对老鼠造成压迫(stress-induced)，使得其血压受到影响， 并可能伤害老鼠的健康, 甚至老鼠死亡. 4. 操作简单: 可设定手动或自动连续测量。 5. 两种操作模式: 模式1: HR(心率)，SBP（收缩压） 模式2: 测量 HR/SBP/MBP(平均血压)/DBP（舒张压，计算值） 6. 连续测量功能：连续测量可以最大到10次，此外为了得到更短的测量时间，也可以连续 快速测量;测量时显示脉波图，测量结果数字显示：收缩压、平均血压、舒张压及心率。 7. 内建快速打印机，可即时打印结果, 屏幕上的数据包括平均值（AV）和标准差（SD）， 1秒钟即可打印输出, 并可用串口将数据传至电脑。 8. 自动序列功能：动物的尾动脉血压能够自动监测，时间间隔可以是1-99分钟。每一次间 隔，能够检测1-10次。该全自动功能可以被输出打印。 9. 时间印记功能: MK-2000ST精确记录每一次BP测量的时间. 10. 硬拷贝功能：屏幕上显示的一切都可以被拷屏打印，只需要摁一下PRTSCREEN按键 10. 串口连接： DCS-2000数据采集软件 11. 动物限制管是由黑色棕丙烯醛基制作，动物在相当安静无干扰的环境下测量 日本MuromachiKikai的大小鼠无创血压仪是目前世界上最好的大小鼠无创血压仪设备， 分为两种不同类型的大小鼠无创血压仪，一种是无需预热的大小鼠无创血压仪， 另外一种是便携性经济型的大小鼠无创血压仪。 SPECIFICATIONS Main Unit  (MK-2000ST) SBP Cuff pressure when pulse reappears MBP Cuff pressure when pulse amplitude becomes largest DBP Calculated value based on SBP and MBP   (3MBP-SBP)/2 Detection LED,  Photo Transistor Inflation Range 0 - 350mmHg Printer Outputs Date, Time, Animal No., Weight, Operation Mode, Interval,   No of Measuring Trials, Raw Data,  Average and S.D. Chart Paper Thermo-sensitive 58 mm Wide x 25m Long Power AC100-130V 50/60Hz Dimensions 210W x 310D x 210 mmH Weight Approx.  8 kg Keyboard Function Keys 33 Dimensions 145W x120D x20 mmH Weight Approx.  0.4 kg The Standard System Comes Supplied with: Main Unit (Printer included) 1 Keyboard 1 Animal Holder (Any one from the list) 1 Cuff-pulse Sensor (Any one from the list) 1 Measuring Stand for Rats 1 TSP-10 Chart Paper (Pack of 5 Rolls) 1 APC-2000 Accessory Case 1 Dust Cover 1 Animal Holders Mouse Holder (3S) 5-10g Mouse Holder (SS) 10-20g Mouse Holder (S) 20-30g Mouse Holder (M) 30-40g Mouse Holder (L) 40-50g Rat Holder (3S) 50-70g Rat Holder (SS) 70-90g Rat Holder (S) 90-150g Rat Holder (SM) 150-210g Rat Holder (M) 210-270g Rat Holder (ML) 270-350g Rat Holder (L) 350-500g Rat Holder (LL) 500-800g Cuff-Pulse Sensors Neo24NH 1.4 - 2g Neo32NH 2 - 5g Neo40NH 5 - 10g Mouse NH 20-50g OB Mouse NH OB Mouse & Mouse 5 - 20g C57 Mouse NH C57BL6 Mouse Hamster (S)NH Approx.  60-80g Hamster (M)NH Approx.  80-120g Hamster (L)NH Approx 120-160g Rat (S)NH 90-210g Rat (M)NH 210-350g Rat (ML)NH 350-500g Rat (L)NH 500-800g 日本MuromachiKikai MK-1030经济型便携式非侵入式无创大小鼠无创血压计 1  支持测量尾部动脉血压 2  测量HR/SBP/MBP/DBP，可以显示在显示屏上 3  HR由10次脉搏决定 4  SBP初始值由加压至25毫米汞柱每秒。放气值大约4-5毫米汞柱每秒，SBP很准确 5  加压可以至400毫米汞柱每秒，SHRSP可以被测量 6  连续测量可达6次 7  动物管是由黑色棕丙烯醛基制作，动物在相当安静无干扰的环境下测量 8  PMB-1030预热测量腔来自于标准系统 9  串口设计连接数据输出打印，DCS-1030数据采集软件 10 A4纸大小，便携 11  操作简单 特性 Main Unit MK-1030 Power AC100-120V 50/60Hz Dimensions 320W x 250D x 80mmH Weight Approx. 5 kg PMB-1030  Preheat&Measuring Box  （Standard Accessory） Heating Method Electric Heater Material Internal:SS External:PCV Sensor Thermistor Display LCD Dimensions 100W x 230D x 150mmH Weight 1.3 kg The Standard System Includes: Main Unit (MK-1030) 1 PMB-1030 Preheat&Measuring Box 1 Animal Holder (any one from the list) 1 Cuff-Pulse Sensor (any one from the list) 1 Animal Holders Rat Holder    (SS) 50 - 90g Rat Holder    (S) 90 - 150g Rat Holder    (SM) 150 - 210g Rat Holder    (M) 210 - 270g Rat Holder    (ML) 270 - 350g Rat Holder    (L) 350 - 500g Mouse Holder     (S) 20 - 30g Mouse Holder     (M) 30 - 40g Mouse Holder     (L) 40 - 50g Cuff-Pulse Sensors Rat (S) 50 - 150g Rat (M) 150 - 270g Rat (L) 270 - 500g Mouse 20 - 50g Optional Extras DCS-1030 Data Collection Software (Windows Version) Printer

To measure blood pressure of small animals such as rats or mice using conventional BP Monitors, animals had to be preheated by some means. However, the advent of MK-2000 has made it possible to measure blood pressure without preheating animals provided that the room temperature is at least 23 degrees C. MK-2000ST is the successor to MK-2000 and has become more compact than ever before.
MK-2000ST is ideal for investigating the effects of drugs on animals.  Once the animals are restrained properly, just press the START key and HR (Heart Rate), SBP (Systolic BP), MBP (Mean BP) and DBP (Diastolic BP, calculated value) will be automatically measured.

It photoplethysmographically measures pulsatile blood pressure of tail arteries. Pulses are stored into the computer memory and the peak levels of the pulses are plotted on the display. X-axis indicates cuff pressure, and Y-axis indicates amplitude of pulses. Using this method, the operator can decrease the risk of selecting artifacts and BP measurement is made more objective compared to the conventional digital display method.

SBP is initially estimated by inflating the cuff at approximately 25 mmHg/sec. During cuff deflation at about 4-5 mmHg/sec, SBP is determined precisely. SBP is defined as the point when the pulse reappears..

Main Features

1. Two operation modes are available.
With Mode 1 HR (Heart Rate) and SBP (Systolic Blood Pressure) are measured. With Mode 2 HR, SBP, MBP (Mean Blood Pressure) and DBP (Diastolic Blood Pressure, calculated value) can be measured.

NOTE：HR (Heart Rate), SBP (Systolic BP), MBP (Mean BP), DBP (Diastolic BP, calculated value)

2. Continuous Measurement Function
Continuous measurement can be performed up to ten (10) times. Further, continuous & rapid measurement can also be performed in order to shorten the measuring time.

3. Rapid Printout Function
Onscreen data including average (AV) and standard deviation (SD), are printed out in about one (1) second by the high-performance thermal printer. Data may also be deleted at the operator's discretion.

4. Colored mice such as C57BL/6,  neonatal mice weighing 1.4 grams, hamsters (anaesthetized) and  guinea pigs (non-anaesthetized), of which blood pressure was so far considered difficult to measure, can be measured.

5. Sick animals can be measured. (e.g.  animal with cardiac infarction)

6. Multi-channel system is no longer needed. Just prepare multiple restrainers, and measurement can be done one after the other.

7.Auto-Sequence Function
Blood pressure of a tail artery of an animal can be monitored automatically at specified time intervals, which can be 1 - 99 minutes in length. At each interval, anywhere from 1-10 measurements can be made. This function invokes a series of procedures automatically--- measurement,  printout of data and transfer to a PC.

8. Time-Stamp Function
The MK-2000ST precisely records the time of each BP measurement.

9. Hard Copy Function
The screen on the display can be captured to the thermal printer using the PrtScreen key.

10. An RS-232C interface is provided on the rear panel of the main unit. Using the optional DCS-2000 Data Collection Software  (Windows Version) the data obtained can be exported to a personal computer and stored as a CSV file.

11. The animal holders are made of dark brown acryl, allowing BP measurement under relatively stress-free conditions.

12. Operation is very simple.

SPECIFICATIONS

---

Correlation between simultaneously recorded blood pressures (BP) from carotid catheter and BP measured with the Model MK-2000 in mice. Good correlation was seen in a wide range of BP, which was induced by i.v. infusions of pressor or depressor agents.

The data was offered courtesy of Prof. Tomoyuki Kuwaki, Ph.D.,
Department of Molecular and Integrative Physiology, Graduate School of Medicine,
Chiba University

Specifications are subject to change without notice.

REFERENCES:

1. Design and production of genetically modified soybean protein with anti-hypertensive activity by incorporating potent analogue of ovokinin (2-7) (FEBS Letters 24860 (2001) 1-5
   Nobuyuki Matoba, Naomi Doyama, Yuko Yamada, Nobuyuki Maruyama, Shigeru Utsumi,  Masaaki Yoshikawa
   Research Institute For Food Science,  Kyoto University,  Uji  Kyoto 611-0011, Japan
   Animal used : SHR
   

2. Design of a Highly Potent Anti-hypertensive Peptide Based on Ovokinin(2-7) (Biosci. Biotechnol. Biochem.,66(6), 1213-1217,2002)
   Yuko Yamada, Nobuyuki Matoba, Hachiro Usui, Kunihiko Onishi, and Masaaki Yoshikawa
   Division of Food Bioscience and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan
   Animal used : SHR and Wky Rat
   

3. Syndecan-4 Deficiency Leads to High Mortality of Lipopolysaccharide-injected Mice (The Journal of Biological Chemistry, Vol. 276, No. 50, Issue of December 14, pp. 47483-47488, 2001)
   Kazuhiro Ishiguroö˜,  Kenji Kadomatsuö÷,  Tetsuhito Kojimaö÷||,  Hisako Muramatsuö,  Mitsunori Iwase||,
   Yasunobu Yoshikai**,  Masamitsu Yanadaö˜,  Koji Yamamoto˜, Tadashi  Matsushita˜,  Masahiko Nishimuraöö
   Kazuo Kusugami˜,  Hidehiko Saito˜ ˜˜,  and Takashi Muramatsuö÷÷
   From the öDepartment of Biochemistry, ˜First Department of Internal Medicine, **Laboratory of Host Defense & Germfree Life, Research Institute for Disease Mechanism and Control, and ööInstitute for Laboratory Animal Research, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550, Japan, the ||Department of  Medical Technology, Nagoya University School of Health Sciences, 1-1-20 Daiko-Minami, Higashi-ku, Nagoya, Aichi, 461-8673,Japan, and the ˜˜Nagoya National Hospital, 4-1-1 Sannomaru, Naka-ku, Nagoya, Aichi, 460-0001, Japan
   

4. Validity and Application of Noninvasive Measurement of Blood Pressure in Hamsters (Exp.Anim. 52(4), 359-363, 2003)
   Kato Y,  Iwase M,  Kanazawa H,  Nishizawa T,  Zhao YL,  Takagi K,  Nagata K,  Noda A,  Koike Y,  Yokota M
   School of Health Sciences, Nagoya University, 1-1-20 Daiko-minami, Higashi-ku, Nagoya 461-8673, Japan.
   

5. Progressive Development of Pulmonary Hypertension Leading to Right Ventricular Hypertrophy Assessed by Echocardiography in Rats (Exp. Anim. 52(4), 285-294, 2003)
   Yosuke KATO1), Mitsunori  IWASE1), Hiroaki  KANAZAWA1),  Natsuki  KAWATA1) ,Yukie YOSHIMORI,1)
   Katsunori HASHIMOTO1),  Toyoharu YOKOI1),  Akiko NODA1),  Kenzo TAKAGI1),  Yasuo KOIKE1),
   Takao NISHIZAWA2),  Masahiko NISHIMURA2), and Mitsuhiro YOKOTA2)
   1)School of Health Science, Nagoya University,1-1-20 Daiko-minami,Higashi-ku, Nagoya 461-8673, and,
   2)Graduate School of Medicine, NagoyaUniversity,65 Tsurumai-cho, Showa-ku,Nagoya 466-8550,Japan
   

6. Role of host angiotensin II type 1 receptor in tumor angiogenesis and growth (The Journal of Clinical Investigation July 2003 Vol.112, No. 1)
   Kimiyasu Egami,1,2 Toyoaki Murohara,1,3,4 Toshifumi Shimada,1,3 Ken-ichiro Sasaki,1,3
   Satoshi Shintani,1,3 Takeshi Sugaya,5 Masahiro Ishii,1,2 Teiji Akagi,1,2 Hisao Ikeda,1,3
   Toyojiro Matsuishi,1,2 and Tsutomu Imaizumi1,3
   1 The Cardiovascular Research Institute,
   2 Department of Pediatrics, and
   3 Department of Internal Medicine III, Kurume University School of Medicine, Kurume, Japan
   4 Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan
   5 Discovery Research Laboratory, Tanabe Seiyaku Co., Osaka, Japan
   Animal used : C57BL6 Mouse
   

7. Growth hormone-releasing peptide can improve left ventricular dysfunction and attenuate dilation in dilated cardiomyopathic hamsters (Cardiovascular Research 61 (2004) 30-38)
   Iwase, M., Kanazawa, H., Kato, Y., Nishizawa, T., Somura, F., Ishiki, R., Hagata, K., Hashimoto, K., Takagi, K., Izawa, H. and Yokota,
   

8. Development of an in vivo Bioassay Method for Allergy-Preventive Substances using HEL-induced Blood Flow Decrease (Biol. Pharm. Bull)
   Kyoko Ishiguro1), Hisae Oku1), Yoshimi Ueda1), Emiko Iwaoka1) and Masaru Kunitomo1)
   1) School of Pharmaceutical Sciences, Mukogawa Women's University
   Animal used : ddY Mouse
   

9. Salt-sensitive hypertension is triggered by Ca²⁺ entry via Na⁺/Ca²⁺ exchanger type-1 in vascular smooth muscle (NATURE MEDICINE Volume 10 Number 11  November 2004)
   Takahiro Iwamoto1,3, Satomi Kita1,5, Jin Zhang2, Mordecai P Blaustein2, Yuji Arai4, Shigeru Yoshida5,
   Koji wakimoto6, Issei Komuro7&Takeshi Katsuragi1
   1 Department of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan.
   2 Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
   3 Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka 565-8565, Japan.
   4 Department of Bioscience, National Cardiovascular Center Research Institute, Osaka 565-8565, Japan.
   5 Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd., Saitama 330-8530, Japan.
   6 Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka 532-8505, Japan.
   7 Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan.
   Animal used : C57BL6 Mouse
   

10. The vascular relaxing effects of sevoflurane and isoflurane are more important in hypertensive than in normotensive rats (Canadian Journal of Anesthesia 51: 979-985, 2004)
    Jingui Yu, MD*, Koji Ogawa, MD, Yasuyuki Tokinaga, MD*, Shizue Iwahashi, MD* and Yoshio Hatano, MD*
    * From the Department of Anesthesiology, and the Surgical Operating Center, Wakayama Medical University, Wakayama City, Japan.
    

11. Angiogenesis and Vasculogenesis Are Impaired in the Precocious-Aging klotho Mouse(American Heart Association  Circulation. 2004;110:1148-1155.)
    Toshifumi Shimada, MD; Yoshiaki Takeshita, MD; Toyoaki Murohara, MD; Ken-ichiro Sasaki, MD;
    Kimiyasu Egami, MD; Satoshi Shintani, MD; Yosuke Katsuda, MD; Hisao Ikeda, MD;
    Yo-ichi Nabeshima, MD; Tsutomu Imaizumi, MD
    From the Cardiovascular Research Institute (T.S., Y.T., T.M., K.-i.S., K.E., S.S., Y.K., H.I., T.I.), Kurume University, Kurume; the Department of Cardiology (T.M.), Nagoya University Graduate School of Medicine, Nagoya; and the Department of Pathology and Tumor Biology (Y.-i.N.), Kyoto University Graduate School of Medicine, Kyoto, Japan.
    

12. Effects of Captoprill on Cardiac and Renal Damage, and Metabolic Alterations in the Nitric Oxide-Deficient Hypertensive Rat (Kidney Blood Pressure Research 2005; 28: 243-250)
    Mahmoud M. Khattab Adel Mostafa Othman Al-Shabanah
    Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh , Saudi Arabia
    

13. Rat Phenome Project: The untapped potential of existing rat strains  (J Appl Physiol 98: 371-379, 2005)
    Tomoji Mashimo, Birger Voigt, Takashi Kuramoto, and Tadao Serikawa
    Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    

14. An X-Ray Diffraction Study on Mouse Cardiac Cross-Bridge Function In Vivo: Effects of Adrenergic ß-Stimulation (Biophysical Journal 90:1723-1728 (2006)
    Ryuji Toh,* Masakazu Shinohara,* Tomofumi Takaya,* Tomoya Yamashita,* Shigeru Masuda,*
    Seinosuke Kawashima,* Mitsuhiro Yokoyama,* and Naoto Yagiy
    *Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine,Kobe 650-0017, Japan; and ySPring-8/JASRI, Sayo, Hyogo 679-5198, Japan
    

15. Prostacyclin agonist with thromboxane synthase inhibitory activity (ONO-1301) attenuates bleomycin-induced pulmonary fibrosis in mice (Am J Physiol Lung Cell Mol Physiol 290: L59-L65, 2006.)
    Shinsuke Murakami,1,2 Noritoshi Nagaya,1,3 Takefumi Itoh,2 Masaharu Kataoka,1 Takashi Iwase,1
    Takeshi Horio,3 Yoshinori Miyahara,4 Yoshiki Sakai,5 Kenji Kangawa,6 and Hiroshi Kimura2
    1Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research
    Institute, Osaka; 2Second Department of Internal Medicine, Nara Medical University, Nara; 3Department of
    Internal Medicine, National Cardiovascular Center, Osaka; 4Department of Cardiac Physiology, National
    Cardiovascular Center Research Institute, Osaka; 5Ono Pharmaceutical Co., Ltd. Research Headquarters,
    Osak, a; and 6Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka, Japan
    

16. Antihypertensive Effect of Angiotensin-Converting Enzyme Inhibitory Peptide Obtained from Hen Ovotransferrin (Journal of the Chinese Chemical Society, 2006, 53, 495-501)
    Nai-Yuan Leea, Juei-Tang Chengb, Toshiki Enomotoc, Ichiro Nakamurad,
    a Livestock Research Institute, Tainan 712, Taiwan, R.O.C.
    b Department Pharmacology, College of Medicine, National Cheng Kung University,
    Tainan 70101, Taiwan, R.O.C.
    c Department of Food Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 9218836, Japan
    d Institute of Animal Experimentation, School of Medicine, Hokkaido University, Sapporo 0608589, Japan
    Animal used : SHR
    

17. Evaluation of Blood Pressure Measured by Tail-Cuff Methods (without Heating) in Spontaneously Hypertensive Rats (Biol. Pharm. Bull. 29(8) 1756-1758 (2006) Vol. 29, No. 8
    KUBOTA Yoko1 UMEGAKI Keizo2 KAGOTA Satomi 3 TANAKA Naoko 4 NAKAMURA Kazuki 5 KUNITOMO Masaru 6 SHINOZUKA Kazumasa 7
    1 Department of Biopharmaceutics, Nihon Pharmaceutical University
    2 National Institute of Health and Nutrition
    3 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
    4 Department of Pharmacology, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare
    5 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
    6 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
    7 Department of Pharmacology, School of Pharmaceutical Sciences, Mukogawa Women's University
    

18. Mechanical ventilation with 40% oxygen reduces pulmonary expressionof genes that regulate lung development and impairs alveolar septation in newborn mice
    (Am J Physiol Lung Cell Mol Physiol 293:1099-1110, 2007. )
    Richard D. Bland, Lucia M. Mokres, Robert Ertsey, Berit E. Jacobson, Shu Jiang, Marlene Rabinovitch,
    Liwen Xu, Eric S. Shinwell, Feijie Zhang, and Matthew A. Beasley
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California
    

19. Mechanical ventilation uncouples synthesis and assembly of elastin and increases apoptosis in lungs of newborn mice.
    (Am J Physiol Lung Cell Mol Physiol 294:L3-L14, 2008. )
    Richard D. Bland, Robert Ertsey, Lucia M. Mokres, Liwen Xu, Berit E. Jacobson, Shu Jiang,
    Cristina M. Alvira, Marlene Rabinovitch, Eric S. Shinwell, and Anjali Dixit
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California