工作原理:
1. 大功率微波加热的方式使得神经组织内的酶(ENZYME)瞬间去活性-灭活,以避免死后变化 (post-mortem changes)持续进展。 2. 此法比用冷冻固定法要来得快速:冷冻法需要10-90秒做深入冷冻去活性,这期间,深部组织的死后变化 已经在进行,因脑表面 的热传导转差。微波法可在更短的时间内使组织完全去活性。 3. 用以测定: * Acetylcholine乙酰胆碱 * Choline 胆碱 * Cyclic AMP 环腺苷酸 * Cyclic GMP 环林鸟苷 * GABA 伽马氨基丁酸 * DOPA 多巴(二羟基苯丙氨酸) * 5-HTP 5-羟色胺酸 * Serotonin 血清素 * Endorphin 内啡肽 * Prostaglandin 前列腺素 * Catecholamines and their metabolites 儿茶酚胺及其代谢物 * Phosphorylated Proteins 磷酸化蛋白质
微波灭活固化可以达到下列标准:
1. 可瞬间将温度升至75-90摄氏度,使脑部快速固定。 2. 大功率微波能量可有效集中在动物的头部。 3. 各种动物都可以达到相同的结果。 4. 对操作人员具备严格的保护,设备相当安全。 5. CE认证,国际规定: 家用微波设备微波泄漏不得超过5毫瓦/立方厘米, 此设备安全标准远远高于国际规定,微波泄漏不超过1毫瓦/立方厘米。
产品特性:
1. 所有控制均位于前触摸屏幕板上; 2. 数字化设置, 使得电源级别设置容易,重复性好; 3. 稳压控制: 很好的控制客户处电压不稳, 电压波动性得到控制; 4. 采用风冷装置,无需水冷,升降温时间快;
5. 整体重量轻,仅为103公斤.
规格
振荡器 |
Panasonic Magnetron 2M265-M11 |
微波输出 |
5kW max. (2-5 千瓦变频) |
震荡频率 |
2.450±30MHz |
冷却要求 |
空气冷却 |
水流量 |
每分钟两升 |
暴露时间 |
0.01-9.99秒 (0.01秒步进) |
暂停时间 |
3 分钟 |
能量功耗 |
8 千瓦 |
频率 |
50 或者 60 Hz |
电源要求 |
190-240 VAC 30A 3相 或者 380-440 VAC 20A 3相 |
外形尺寸 |
750 W x 550 D x 1,275 H 厘米 |
重量 |
大约 103公斤 |
APPLICATOR HEAD 适用的动物管
TAW-174P |
WJM-24 for Mouse 15-20g WJM-28 for Mouse 20-40g |
TAW-424SP |
WJR-S for Rat 150-250g |
TAW-424MP |
WJR-M for Rat 250-400g WJR-L for Rat 400-500g |
发表文献:
Unambiguous assignment of the H3S and H3R deuterations of cerebral (2-13C) glutamate by 13C NMR at 18.8 tesla. TB Rodrigues, IR Violante, S … - Magnetic Resonance in …, 2010 - interscience.wiley.com ... At the end of the infusions, cerebral metabolism was arrested using a 5- kW microwave fixation system, the brain being rapidly removed from the skull and immediately frozen in liquid N2. ...
Brain glutamine synthesis requires neuronal-born aspartate as amino donor for glial glutamate formation B Pardo, TB Rodrigues, L Contreras, M … - Journal of Cerebral …, 2010 - nature.com ... regulated circulating water bath. Fifteen minutes after 13 C-label administration, the metabolism was arrested using a high-power (5 kW) focused microwave fixation system The brain was ...
Delayed Increase of Brain Noradrenaline After Acute Footshock Stress in Rats
T Shinba, N Ozawa, M Yoshii, K Yamamoto - Neurochemical research, 2010 - Springer ... 4.5–5 kW, 1.4 s) using a small animal microwave applicator... Immediately after microwave radiation, the brains were harvested and put onto an ice-chilled Petri dish ... Moroji T, Takahashi T, Ogura K, Toishi T, Arai S (1977) Rapid fixation of rat ...
Altered Affect, Monoamine Transmitters and Bioenergetic Homeostasis of Alpha-synuclein-transgenic Mice, in the Presence and Absence of Endogenous Alpha- EM Cumyn - 2010 - tspace.library.utoronto.ca ... 36 3.7 Open field test..... 37 3.8 Microwave fixation and dissection of brain tissue..... 37 3.9 Fresh dissection and flash-freezing of brain tissue..... 40 ...
Brainstem Concentrations of Cholesterol are not Influenced by Genetic Ablation of the Low-Density Lipoprotein Receptor
AY Taha, CT Chen, Z Liu, JH Kim, HTJ Mount, … - Neurochemical …, 2009 - Springer ... At 7 weeks of age, the mice were euthanized with a high-energy head-focused microwave fixation system (6 kW, 0.88–0.99 s, Muromachi brain fixation system in order to stop brain lipid metabolism [14, 15]. ...
Detection of reduced GABA synthesis following inhibition of GABA transaminase using in vivo magnetic resonance signal of [13C] GABA C1
J Yang, C Johnson, J Shen - Journal of neuroscience methods, 2009 - Elsevier ... After in vivo MRS data acquisition of gabaculine-treated rats infused with [2,5- 13 C 2 ]glucose and, unlabeled glucose, the rats were euthanized using a microwave fixation device (Model TNW-6402C, 5 kW, 1.5 s). Subsequently, the rat ... |