大鼠/小鼠脑缺血MCAO模型尼龙栓线(线栓)
发布时间:2009-11-03
大鼠/小鼠脑缺血MCAO模型尼龙栓线(线栓)


Doccol Made a Difference in Stroke Modeling

Doccol MCAO Sutures Reduce SD to 5% of Its Corresponding Mean Value for Infarction Volume

In the stroke model literature, two types of MCAO models are most frequently used, which are the Koizumi model (Koizumi et al. 1986), and the Longa model (Longa et al. 1989). The Koizumi model suggested the use of silicon rubber-coated monofilaments as occludors whilst the Longa model used flame-blunted monofilaments. It turned out that silicon rubber-coated monofilaments are superior to flame-blunted monofilaments for producing consistent ischemic brain injuries. A study (Tsuchiya et al. 2003) showed that using flame-blunted monofilaments for inducing MCA occlusion caused 40% occurrence of subarachnoid hemorrhage, and the standard deviation for infarction volume was greater than its corresponding mean value. Another technical paper specifically compared the effects of suture types on the infarct consistency and showed that silicon rubber-coated monofilaments were superior to flame-blunted ones, producing consistent infarction; and this was true even with inexperienced surgeons. (Shimamura et al. 2006a) In the mouse intraluminal model, SAH rate could reach 40% if uncoated heat-blunted monofilaments being used; and such a modeling method resulted in the deviation for infarction volume being larger than 50% of its corresponding mean value. (Tsuchiya et al. 2003) The poly-L-Lysine coated monofilament seemed could only increase the mortality (to a rate of 60%), without any benefits for reducing infarct variation. (Huang et al. 1998)

The mechanism behind the outcome variation is the frequent occurrence of insufficient occlusion, premature reperfusion, and filament dislodgement. Doccol MCAO sutures have a sufficient length of coated surface, which is cylindrical in shape, elastic, and smooth. Such a physical property of Doccol MCAO sutures is significantly effective in reducing the occurrence of the insufficient occlusion, the premature reperfusion, and the mono filament dislodgement. The standard deviation for infarction volume, when home-made silicon rubber-coated monofilaments being used, is around 30% of its corresponding mean value both in rats (Schmid-Elsaesser et al. 1998) and in mice (Shah et al. 2006). The use of Doccol MCAO sutures has been reported to be able to achieve a much better result. For examples, a 15-min occlusion by using home-prepared monofilaments usually produces no apparent ischemic damage whilst a consistent caudate infarction can be achieved when Doccol MCAO sutures being used; (Pignataro et al. 2007a) The standard deviation for infarction volume is around 10% to 20% of its corresponding mean values for 60-min transient to 24-h permanent occlusions in rats, (Candelario-Jalil et al. 2008; Khan et al. 2006; Liu et al. 2006; Shimamura et al. 2006b; Solaroglu et al. 2006; Tsubokawa et al. 2007; Tsubokawa et al. 2006a; Tsubokawa et al. 2006b) and around 5-10% of its corresponding mean value for the 60-min occlusion in mice, (Kleinschnitz et al. 2007; Maysami et al. 2008; Pignataro et al. 2007b; Pignataro et al. 2007c) when Doccol MCAO sutures being used. Some variation in infarct volumes may be contributed by the differences of experimental design and the selection of the right-sized monofilaments.

Doccol has been serving the stroke research field by providing MCAO sutures (silicon rubber-coated monofilaments) since 2004. Our delicate silicon rubber-coated monofilaments are specially designed and manufactured for inducing the intraluminal filament model of middle cerebral artery occlusion (MCAO), which has been widely used for in vivo experimental stroke studies. Using Doccol MCAO sutures for inducing stroke models will improve your model quality by reducing the occurrence of both the incomplete occlusion and the intracranial bleeding. With Doccol MCAO sutures, you will be able to achieve a highly consistent infarct as well as a low mortality rate. In addition to the super product quality, Doccol provides MCAO sutures with a great variety that was designed to meet the diverse demand from stroke investigators. For many years our MCAO sutures have been preferably selected by stroke investigators for inducing stroke models. We are proud to say that our clients are top-ranked universities, highly prestigious research institutes, and world-leading pharmaceutical companies from the United States,Canada, most European countries,Australia,Japan,South Korea, andIndia. Using Doccol MCAO sutures for inducing MCAO models has been reported to be able to reduce the standard deviation to 5% of its corresponding mean value as for infarction volume; and this modeling method is becoming a new standard for ensuring the highest quality of MCAO models. For more information please read Doccol Made a Difference in Stroke Modeling.

The following are selected publications that refer to our products:

1. Kim E, Tolhurst AT, Qin LY, Chen XY, Febbraio M, Cho S.

CD36/fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury. J Neurosci. 2008 Apr 30;28(18):4661-70.

2. Candelario-Jalil E, Muñoz E, Fiebich BL.

Detrimental effects of tropisetron on permanent ischemic stroke in the rat. BMC Neurosci. 2008 Feb 6;9:19.

3. Terao S, Yilmaz G, Stokes KY, Ishikawa M, Kawase T, Granger DN.

Inflammatory and injury responses to ischemic stroke in obese mice. Stroke. 2008 Mar;39(3):943-50.

4. Varga-Szabo D, Braun A, Kleinschnitz C, Bender M, Pleines I, Pham M, Renné T, Stoll G, Nieswandt B.

The calcium sensor STIM1 is an essential mediator of arterial thrombosis and ischemic brain infarction. J Exp Med. 2008 Jul 7;205(7):1583-91.

5. Maysami S, Lan JQ, Minami M, Simon RP.

Proliferating progenitor cells: a required cellular element for induction of ischemic tolerance in the brain. J Cereb Blood Flow Metab. 2008 Jun;28(6):1104-13.

6. Atochin DN, Wang A, Liu VW, Critchlow JD, Dantas AP, Looft-Wilson R, Murata T, Salomone S, Shin HK, Ayata C, Moskowitz MA, Michel T, Sessa WC, Huang PL.

The phosphorylation state of eNOS modulates vascular reactivity and outcome of cerebral ischemia in vivo. J Clin Invest. 2007 Jul;117(7):1961-7.

7. Tsubokawa T, Jadhav V, Solaroglu I, Shiokawa Y, Konishi Y, Zhang JH.

Lecithinized superoxide dismutase improves outcomes and attenuates focal cerebral ischemic injury via antiapoptotic mechanisms in rats.Stroke. 2007 Mar;38(3):1057-62. .

8. Zhang Z, Yang X, Zhang S, Ma X, Kong J.

BNIP3 upregulation and EndoG translocation in delayed neuronal death in stroke and in hypoxia.Stroke. 2007 May;38(5):1606-13.

9. Sayeed I, Wali B, Stein DG.

Progesterone inhibits ischemic brain injury in a rat model of permanent middle cerebral artery occlusion. Restor Neurol Neurosci. 2007;25(2):151-9.

10. Kleinschnitz C, Pozgajova M, Pham M, Bendszus M, Nieswandt B, Stoll G.

Targeting Platelets in Acute Experimental Stroke. Impact of Glycoprotein Ib, VI, and IIb/IIIa Blockade on Infarct Size, Functional Outcome, and Intracranial Bleeding. Circulation. 2007 May;115(17):2323-30.

11. Pignataro G, Simon RP, Xiong ZG.

Prolonged activation of ASIC1a and the time window for neuroprotection in cerebral ischaemia. Brain. 2007 Jan;130(Pt 1):151-8.

12. Pignataro G, Studer FE, Wilz A, Simon RP, Boison D.

Neuroprotection in ischemic mouse brain induced by stem cell-derived brain implants. J Cereb Blood Flow Metab. 2007 May;27(5):919-27.

13. Cho S, Szeto HH, Kim E, Kim H, Tolhurst AT, Pinto JT.

A novel cell-permeable antioxidant peptide, SS31, attenuates ischemic brain injury by down-regulating CD36. J Biol Chem. 2007 Feb 16;282(7):4634-42.

14. Pignataro G, Simon RP, Boison D.

Transgenic overexpression of adenosine kinase aggravates cell death in ischemia. J Cereb Blood Flow Metab. 2007 Jan;27(1):1-5.

15. Kleinschnitz C, Stoll G, Bendszus M, Schuh K, Pauer HU, Burfeind P, Renné C, Gailani D, Nieswandt B, Renné T.

Targeting coagulation factor XII provides protection from pathological thrombosis in cerebral ischemia without interfering with hemostasis. J Exp Med. 2006 Mar 20;203(3):513-8. Epub 2006 Mar 13.

16. Solaroglu I, Tsubokawa T, Cahill J, Zhang JH.

Anti-apoptotic effect of granulocyte-colony stimulating factor after focal cerebral ischemia in the rat. Neuroscience. 2006 Dec 28;143(4):965-74.

17. Shimamura N, Matchett G, Tsubokawa T, Ohkuma H, Zhang J.

Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model. J Neurosci Methods. 2006 Sep 30;156(1-2):161-5.

18. Khan M, Jatana M, Elango C, Paintlia AS, Singh AK, Singh I.

Cerebrovascular protection by various nitric oxide donors in rats after experimental stroke. Nitric Oxide. 2006 Sep;15(2):114-24.

19. Shimamura N, Matchett G, Yatsushige H, Calvert JW, Ohkuma H, Zhang J.

Inhibition of integrin alphavbeta3 ameliorates focal cerebral ischemic damage in the rat middle cerebral artery occlusion model. Stroke. 2006 Jul;37(7):1902-9.

20. Liu S, Liu W, Ding W, Miyake M, Rosenberg GA, Liu KJ.

Electron paramagnetic resonance-guided normobaric hyperoxia treatment protects the brain by maintaining penumbral oxygenation in a rat model of transient focal cerebral ischemia. J Cereb Blood Flow Metab. 2006 Oct;26(10):1274-84.

21. Tsubokawa T, Yamaguchi-Okada M, Calvert JW, Solaroglu I, Shimamura N, Yata K, Zhang JH.

Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats. J Neurosci Res. 2006 Sep;84(4):832-40.

22. Tsubokawa T, Solaroglu I, Yatsushige H, Cahill J, Yata K, Zhang JH.

Cathepsin and calpain inhibitor E64d attenuates matrix metalloproteinase-9 activity after focal cerebral ischemia in rats. Stroke. 2006 Jul;37(7):1888-94.

大鼠/小鼠脑缺血MCAO模型尼龙栓线(线栓).

产品厂家:Doccol Corporation

规格标准(大鼠不同体重用不同规格的栓线)
价格,

Animal Body Weight (g)

Suggested Diameters (mm)

Filament USP “0” #

Available Coating Lengths (mm)

Using Search Function

My Special Design subcategories

15-20

0.17,0.19

70

2-10

Search “70”, “7017”, “7019”

“70SPRe”

20-25

0.21, 0.22

70, 60

2-10

Search “70”, “60”

“70SPRe”, “60SP”, and “60SPRe”

25-30

0.22, 0.23

70, 60

2-10

Search “70”, “60”

“70SPRe”, “60SP”, and “60SPRe”

30-35

0.23

60

2-10

Search “60”

“60SP”, and “60SPRe”

<200

0.31, 0.33, 0.29

50

2-6*

Search “50”

“50SPRe”

<200-250

0.33, 0.35

50, 40

2-6*

Search “50”, “40”; “5033”, “4035”

“50SPRe”, “40SP”, and “40SPRe”

250-280

0.35, 0.37

40

2-6*

Search “40”; “4035”, “4037”

“40SP”, and “40SPRe”

280-330

0.37, 0.39

40

2-6*

Search “40”; “4037”, “4039”

“40SP”, and “40SPRe”

330-400

0.41

40, 30

2-6

Search “40”, “30”

“40SP”, “40SPRe”, and “30SP”

>400

0.43

30

2-6

Search “30”

“30SP”


上一篇:没有了
下一篇:没有了