动物行为LABORAS及轨跡超音波分析仪SONOTRACK系統介紹
发布时间:2008-01-11
全自动行为分析之新技术 – 动物行为LABORAS及轨跡超音波分析仪SONOTRACK系統介紹

 

全自动行为分析之新技术 – 动物行为LABORAS及轨跡超音波分析仪SONOTRACK系統介紹
 
Metris LABORAS系統是一套利用动作感应器及震动偵測的原理,无关实验環境的採光,可以在全黑的環境下运作,且不需要要求动物的顏色,即可自动偵測几項常見的动物行为,包括:Locomotion、Resting、Rearing、Climbing、Grooming、Drinking、Eating,及Stereotyped Behaviors等。

   LABORAS的系統,除了可以自动偵測动物的行為之外,在同一偵測原理下,尚可偵測动物的軌跡,其准确度可达1mm!所以可以提供动物在飼养笼中,自由活动的轨跡、行进的路徑及距離、行进的速度、平均、最大值,以及偏好位置的分佈。
      Laboras is a sysem that automatically recognizes behaviors of rats
      and mice. This includes normal behaviors (such as locomotion,
      immobility, grooming, rearing, eating, drinking) but also special
      stereotypical behaviors (such as scratching, Wet Dog Shakes,
      Head Shakes, Seizures etc.). Laboras also determines several
      tracking parameters (such as position, speed, distance, number
      of circles etc.)
      (see more at www.metris.nl/en/products/laboras)
  

LABORAS is a measurement system which is used to recognize automatically behaviours of small rodents (mice and rats). The system is currently the only system in the market which is able to determine a large number of different behaviours without traditional human observation. The following behavioural elements can be distinguished: climbing, drinking, eating, grooming, immobility, locomotion and rearing. In addition the system determines the following tracking parameters: position, speed, maximum speed, traveled distance and position distribution. Recently, LABORAS release 2 was launched with an improved user interface and faster processing capability.
Examples of application areas: Ethology, (Safety) Pharmacology, Dermatology, CNS and Toxicology.


Download the LABORAS promotion video now

Laboras Specifications

LABORAS is designed for and with the help of laboratory animal researchers, which has resulted in a system that is easy to use and matches your research needs.

Below we have summarized the main functions and performance of the Laboras equipment. Laboras is a modular system, so it is possible to configure the system with different combinations of functions and to upgrade the system at a later moment.

 

Standard Behavior detection functionality

  • Resting (immobility):
    Movements are absent while the animal is in a sitting or lying position.
    Very short movements (e.g. turning over while sleeping ) are not considered as an interruption.
  • Locomotion:
    Activities such as walking, running, jumping.
  • Rearing (rats only):
    The animal is standing upright on its hind legs (mostly against a side wall of the cage).
  • Eating:
    The animal eats food pellets while standing upright, gripping the bars of the food hopper or the climbing grid in the enriched cages. While the animal is climbing or hanging, the hind legs or tail may touch the floor or side wall of the cage.
  • Drinking:
    The animal stands upright gripping the water bottle and licking water.
  • Grooming:
    The animal is shaking, scratching, wiping or licking its fur, snout, ears, tail or genitals.
  • Climbing (mouse only):
    Climbing and hanging on the bars of the wire cage lid or food hopper or on the climbing grid in the enriched cages. While the animal is climbing or hanging, the hind legs or tail may touch the floor or side wall of the cage.
  • Undefined:
    All behaviours not classified in one of the previous categories.

 

Tracking functionality

  • Position:
    The position of the animal relative to the center of the cage. (resolution = 1 mm; accuracy better than 5 mm)
  • Distance traveled:
    The total distance traveled by the animal during the test. (resolution = 1 cm; accuracy better than 10 cm/hr)
  • Maximum speed:
    The highest velocity of the animal during the test. (resolution = 0.1 cm/s; accuracy better than 0.5 cm/s)
  • Position distribution:
    Graphical presentation showing the preference of the animal for certain spots.
  • Circling: NEW!
    Number of circles (clockwise and counter clockwise) that the animal makes during test. Two types of circling are scored "wide circling" and "turning around the axis". In addition average diameter of circle, average time in circle, average speed in circle are calculated.

Special Behavior detection functionality

  • Scratching (in mice): NEW!
    Repetitive fast movement of the hindlimb, rubbing the side of the body, neck and face.
    Spontaneous scratching epsisodes are generally shorter than a second. Drug induced scratching or scratching because of skin diseases can last for seconds or even minutes.
  • Purposeless Chewing (in rats): NEW!
    Chewing movements in the absence of food. Generally consisting of large chewing movements (head moving) and small chewing movements (lip movement only)
  • Hindlimb licking (formalin test)
    The animal licks the hindlimb as a pain response to a formalin injection in the hindlimb. The three phase response consisting of Phase-I, Q(uiet)-phase and Phase-II are accurately determined by LABORAS.
  • Wet Dog Shakes (in rats): NEW!
    A paroxysmic or quivering shudder of the trunk (body) up to the shoulders of the rat, consisting of a series of radial movements of the body, during which the trunk rotates along the center axis of the body. The quivering shudder seems to roll over the body of the animal from the shoulder to the tail.
    Depending of the intensity and type of WDS the quivering shudder sometimes extends beyond the shoulder of the animal, including the neck and sometimes the head.
  • Seizures (in mice): NEW!
    Several types of strong seizures can be detected. Amongst others: Barrel roll seizures, tonic clonic seizures and seizures P4 and P5 (scored on the Racine scale).

 

User Interface

  • GLP compatible user interface and data management The software complies to the GLP requirements for software used in laboratory instruments
  • Easy to use interface:
    Straight forward user interface with pull-down menus and experiment navigation tree
  • extensive and complete experiment data administration
    All experiment data can be entered in the administration software module of LABORAS. The experiment data is added to the test results. Test sessions and related data being part of one experiment can be kept together in one experimental design.

Output definitions

  • time tagged behaviour files and position files
  • test result summaries over user definable windows:
    Including Behavior Durations and Behavior Frequencies
  • export of test results in several commonly used formats:
    MS-Excel, SPSS, SAS/JMP, etc.


 

 

SONOTRACK ® 
 
  另外,像老鼠發出的超音波,雖不為人耳所能听到,但是能夠代表其当时心理舒適或痛苦的狀況,甚至在行為表現之前即已在声音上表達出來了!這对于老鼠在環境适应性、或做痛觉实验、药物实验,以及驚嚇、压力、沮喪、害怕等等实验,都可以在声音上反应其結果。
        
    但是,超音波的声波,並非人耳所能听到的!Metris SONOTRACK的系統, 採用Time Expansion的方法,可以測到20KHz到100KHz的全頻超音波,並將其转为人耳可以听到的声音,可以存成電腦上wav的檔案格式,方便了解老鼠超音波的声波。
 
     Sonotrack records, analyzes and plays back ultrasound vocalizations
      (USV calls) of animals. It can automatically detect the calls and calls
      can be played back to the animal.  The system can automatically
      determine the number of calls in different frequency bands, the min.,
      max. and average duration of calls, the min., max. and average frequency
      of calls, etc.
      (see more at www.metris.nl/en/products/sonotrack)

SONOTRACK is an advanced non-invasive measurement system to monitor ultrasonic sounds produced by animals. By using a dedicated microphone and hardware the system is able to pick up ultrasonic vocalizations (USV) of animals generated at frequencies between 15 kHz and 100 kHz.

The system not only records and converts the ultrasounds in a for humans audible signal but also provides several graphical presentations of the recorded sounds and an user adjustable Ultrasound Call counter. This counter determines the number of calls in user definable frequency bands, the start time and duration of each call as well as the mean frequency of each call.
Examples of application area: Ethology, CNS and Pharmacology, Bio Acoustics, Pain, Animal welfare studies.

 

SLEEPSIGN for Animal ® This is used for analysis of Sleep patterns.
Powerful sleep analysis software for manual and automatic sleep stage scoring. Recognizes sleep stages such as REM, Wake, etc.  Investigates sleep disorders in animals
 
 

LABORAS Publications

2009

bullet2.gif Bison, S.; Carboni, L.; Arban, R.; Bate, S.; Gerrard, P.A.; Razolli, M.;
Differential behavioral, physiological, and hormonal sensitivity to LPS challenge in rats
International Journal of Interferon, Cytokine and Mediator Research 2009:1, page 1-13 (2009) Full article

2008

bullet2.gif Woolley, M.L.; Pemberton, D.J.; Bate S.; & Corti, C.; Jones, D.N.C.
The mGlu2 but not the mGlu3 receptor mediates the actions of the mGluR2/3 agonist, LY379268, in mouse models predictive of antipsychotic activity
Psychopharmacology, (2008) 196:431–440 Full article
bullet2.gif Wood, N.I.; Goodman, A.O.; Van der Burg, J.M.; Gazeau, V.; Brundin, P.; Björkqvist, M.; Petersén, A.; Tabrizi, S.J.; Barker, R.A.; Morton, A.J.;
Increased thirst and drinking in Huntington's disease and the R6/2 mouse
Brain Research Bull, 76 (1-2):70-9 (2008) Full article
bullet2.gif Inglis, J.J.; McNamee, K.E.; Chia, S.; Essex, D.; Feldmann, M.; Williams, R.O.; Hunt,S.P.; Vincent, T.
Regulation of pain sensitivity in experimental osteoarthritis by the endogenous peripheral opioid system
Arthritis & Rheumatism Vol. 58, Issue 10, Pages 3110 -3119 (Sept. 2008) Details
bullet2.gif Avsaroglu, H.; Sommer, R.; Hellebrekers, L.J.; Van Zutphen, L.F.M.; Van Lith, H.A.;
The effects of buprenorphine on behaviour in the ACI and BN rat inbred strains
Laboratory Animals, 42 (2008), Page 171-184 Detail
bullet2.gif Van der Burg, J.J.M.; Bacos, K.; Wood, N.I.; Lindqvist, A.; Wierup, N.; Woodman, B.; Wamsteeker, J.I.; Smith, R.; Deierborg, T.; Kuhar, M.J.; Bates, G.P.; Mulder, H.; Erlanson-Albertsson, C.; Morton, A.J.; Brundin, P.; Petersén, A.; Björkqvista, M.
'Increased metabolism in the R6/2 mouse model of Huntington's disease'
Neurobiology of Disease, 29 (2008), Page 41-51 Full article
bullet2.gif Franěk, M.; Vaculín, Š.; Rokyta, R.
'Changes of locomotor activity in animal models of neuropathic pain'
12th World Congress on Pain, Glasgow, Scotland (August 2008) Poster
bullet2.gif Hedenqvist, P.
'Anaesthesia and analgesia for surgery in rabbits and rats, a comparison of the effects of different compounds'
Doctorial Thesis, Karolinska Institutet, Stockholm (2008), ISBN: 978-91-7357-578-2 Full report

Sonotrack Publications

2009

bullet2.gif Takahashi, A.; Yap, J.J.; Zitzman Bohager, D.; Faccidomo, S.; Clayton, T.; Cook, J.M.; Miczek, K.A.
Glutamatergic and GABAergic modulations of ultrasonic vocalizations during maternal separation distress in mouse pups
Psychopharmacology (2009) 204:61–71 Full article

2005..2008

bullet2.gif Hansen, E.; Agmo, A.;
Precopulatory Ultrasonic Vocalizations in Rats: Noise or Communications?
Poster University of Tromsoe (2006) Poster
bullet2.gif Portfors, C.V.;
Types and Functions of Ultrasonic Vocalizations in Laboratory Rats and Mice
Journal of the American Association for Laboratory Animal Science (2007) Full article
bullet2.gif

Oliveira, A.R.; Barros, H.M.T.
'Ultrasonic Rat Vocalizations During the Formalin Test: A Measure of the Affective Dimension of Pain?'
Anesthesia Analg 2006;102:832–9 (2006) Full article

bullet2.gif Metris b.v. / Dr. Ellen Molewijk
'High Quality Simultaneous Measurements of Rodents Behaviour, Tracking, Physiology, Sleep Stages and Ultrasounds'
Touch Briefings, Future Drug Discovery 2006, (report January 2006) Full article
bullet2.gif

Holy, T.E.; Guo, Z.
'Ultrasonic Songs of Male Mice'
PLoS Biology Dec. 2005, Vol 3, Issue 12, e386 (2005) Full article

bullet2.gif Metris b.v. / Dr. Ellen Molewijk
'High Quality Measurements of Rodents Behaviour, Tracking and Ultrasounds Using LABORAS and SONOTRACK'
Touch Briefings, Future Drug Discovery 2005, (report January 2005) Full article
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